POnebyOne™ FREE Bicistronic Mammalian Expression Kit

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Life Sciences

POnebyOne™ FREE Bicistronic Mammalian Expression Kit

Sale Price:

$577.80

Available on

Oct 1 2019 12:00AM

Catalog ID:

MEF020

In Stock:

Yes

Size:

20 rxn (1 µg)

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Description	POnebyOne™ FREE Bicistronic Mammalian Expression Kit
Catalog No.	MEF020
Advantages & Features	Complete solution: a cloning vector to clone and produce two proteins of interest. Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (for example, a protein of interest and reporter). Highly efficient: tested with up to 4 kb inserts. Accurate: proven performance for most common cell lines. Flexible: allows transfection in difficult cell lines. Convenient: available with different resistance marker cassettes. Reporter checking: stoichiometric amount of your protein of interest and a reporter protein. Risk-free: product covered by our Quality 100% Guarantee.
Specifciations	Cytomegalovirus Promoter: 232-887 Multiple Cloning site 1 (MCS 1): 895-967 T2A from Thosea asigna virus: 968-1030 Multiple Cloning site 2 (MCS 2): 1031-1065 BGH Polyadenylation Sequence: 1082-1306 f1 Origin: 1362-1780 Neomycin Resistance Cassette: 1785-3288 Neomycin Resistance Gene (ORF): 2190-2981 pUC Origin: 3671-4341 Ampicillin Resistance Cassette (complementary strand): 4486-5480 Ampicillin Resistance Gene (ORF): 4489-5346
Applications	Protein Expression of intracellular, extracellular or transmembrane higher cells in an equimolecular to a surface marker that allows quantification.
Quality Control	Cloning of SEAP open reading frame and testing phosphatase alkaline activity in mammalian cells.
Download Documentation	https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2018/05/pOnebyOne%E2%84%A2-FREE.pdf
Safety Statements	This product is developed, designed and sold *exclusively for Research purposes and in vitro* use only (RUO)**. The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

DISCLAIMER:

For research use only. We do not sell to patients and pharmacies