Brand |
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Description |
pSpark® Universal DNA Cloning Kit
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Catalog No. |
C0019 |
Advantages & Features |
Compatible with Blunt and TA DNA cloning: it is composed by pSpark® II and pSpark® TA DNA Cloning vectors.
- Convenient: ideal for a broad range of PCR fragments cloning applications.
- Versatile: compatible with any DNA Polymerase.
- Risk-free: product covered by our Quality 100% Guarantee.
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Download Documentation |
MSDS: https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/08/MSDS-pSpark-DNA-Cloning-Kits8.pdf
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Application
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- Cloning of high fidelity PCR amplified products into pSpark® II Blunt DNA cloning vector.
- Cloning of non-proofreading PCR fragments into pSpark® TA DNA Cloning vector.
- Production of ssDNA.
- In vitro transcription from T7/SP6 dual-opposed promoters.
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Quality Control
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- Functional test using a 1.0 kb PCR fragment.
- Functional test using a 600 pb PCR fragment.
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Storage
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- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
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Safety Statements
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This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website. |
Advise
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- Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above -20 °C inactivates the enzyme.
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