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POnebyOne™ FREE Bicistronic Mammalian Expression Kit
POnebyOne™ FREE Bicistronic Mammalian Expression Kit
Sale Price:
$577.80
Available on
Oct 1 2019 12:00AM
Catalog ID:
MEF020
In Stock:
Yes
Size:
20 rxn (1 µg)
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Description  POnebyOne™ FREE Bicistronic Mammalian Expression Kit   
Catalog No. MEF020
Advantages & Features
  • Complete solution: a cloning vector to clone and produce two proteins of interest.
  • Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (for example, a protein of interest and reporter).
  • Highly efficient: tested with up to 4 kb inserts.
  • Accurate: proven performance for most common cell lines.
  • Flexible: allows transfection in difficult cell lines.
  • Convenient: available with different resistance marker cassettes.
  • Reporter checking: stoichiometric amount of your protein of interest and a reporter protein.
  • Risk-free: product covered by our Quality 100% Guarantee.
Specifciations
  • Cytomegalovirus Promoter: 232-887
  • Multiple Cloning site 1 (MCS 1): 895-967
  • T2A from Thosea asigna virus: 968-1030
  • Multiple Cloning site 2 (MCS 2): 1031-1065
  • BGH Polyadenylation Sequence: 1082-1306
  • f1 Origin: 1362-1780
  • Neomycin Resistance Cassette: 1785-3288
  • Neomycin Resistance Gene (ORF): 2190-2981
  • pUC Origin: 3671-4341
  • Ampicillin Resistance Cassette (complementary strand): 4486-5480
  • Ampicillin Resistance Gene (ORF): 4489-5346
Applications
  • Protein Expression of intracellular, extracellular or transmembrane higher cells in an equimolecular to a surface marker that allows quantification.
Quality Control
  • Cloning of SEAP open reading frame and testing phosphatase alkaline activity in mammalian cells.
Download Documentation https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2018/05/pOnebyOne%E2%84%A2-FREE.pdf
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

pOnebyOne™ FREE Bicistronic Mammalian Expression Kit

DISCLAIMER:
For research use only. We do not sell to patients and pharmacie
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