• Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
• Great efficiency: over hundreds positive colonies with few nanograms of insert.
• High stability: eliminates cloning bias or pitfalls.
• Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
• Powerful: clone from less than 1 ng/kb to up to 14 kb; obtain 4-fold more positive colonies using 3-fold less DNA insert.
• Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding «suicide» strategies from toxic genes.
• Great versatility: compatible with any protocol, proofreading Polymerase, Competent Cells, ligation time or primers.
• Flexible: ligation time from 10 minutes to overnight.
• Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
• High cost-saving:  reduces your cloning costs as no expensive phosphorylated primers are needed.
• Eliminates positive selection vector.
• Risk-free: product covered by our Quality 100% Guarantee.
  

 Manual: https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/08/pSpark-DNA-Cloning1.pdf

MSDS: https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/08/MSDS-pSpark-DNA-Cloning-Kits1.pdf

• General cloning.
• Clone PCR fragments included in a low amount.
• Cloning of PCR products amplified with High Fidelity polymerases.
• Cloning of PCR fragments generated with a blend of polymerases.
• Production of ssDNA.
• Blue/white screening for recombinants.
• In vitro transcription from T7/SP6 dual-opposed promoters.

• Functional test using a 1.0 kb PCR fragment.

• Shipped in: Gel pack.
• Storage: -20 ºC (NON Frost-Free Freezer).
• Shelf Life: 24 months from the date of purchase, if it is properly stored.

• Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above    -20 °C inactivates the enzyme.