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PSpark® Done DNA Cloning Kit
PSpark® Done DNA Cloning Kit
Available on
Oct 1 2019 12:00AM
Catalog ID:
C0006-S
In Stock:
Size:
10 rxn
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pSpark® Done DNA Cloning Kit

Catalog No.
C0006-S
Advantages & Features

 

  • Optimized: recognition sites for Not I and EcoR I at either side for the insertion of the cloning point.
  • Flexible: allows removing the desired insert DNA with other restriction digestion.
  • Unprecedented efficiency: more than 2,500 positive colonies expected under optimal conditions.
  • Easy-to-use: eliminate screening of recombinants due to its its minumum background (lower than 1%).
  • Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating.
  • Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert.
  • High stability: eliminates cloning bias or pitfalls.
  • Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
  • Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
  • Eliminates positive selection vector.
  • Cost avoidance: removes expensive primer phosphorylation use.
  • Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
  • Risk-free: product covered by our Quality 100% Guarantee.

 

Download Documentation

 Manual: https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/08/pSpark-DNA-Cloning5.pdf

 MSDS: https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/08/MSDS-pSpark-DNA-Cloning-Kits5.pdf

Application
  • Cloning of High Fidelity PCR amplified products.
  • Production of ssDNA.
  • Blue/white screening for recombinants.
  • In vitro transcription from T7/SP6 dual-opposed promoters.
  • One restriction enzyme allows gene fragment excision.
Quality Control
  • Functional test using a 1.0 kb PCR fragment.
Storage
  • Shipped in: Gel pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Advise
  • Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above    -20 °C inactivates the enzyme.

pSpark® Done DNA Cloning Kit

DISCLAIMER:
For research use only. We do not sell to patients and pharmacies
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