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TA Cloning Kits
TA Cloning Kits
Sale Price:
$440.00
Available on
Oct 1 2019 12:00AM
Catalog ID:
C0021
In Stock:
Yes
Size:
20 rxn
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pSpark® TA Done DNA Cloning Kit

Catalog No.
C0021
Advantages & Features
  • Convenient: recognition sites for EcoRI and NotI flanking the insertion site.
  • Flexible: allows removing the desired insert DNA with other restriction digestion.
  • Efficient: more than 600 white positive colonies expected under optimal conditions.
  • Stable: without cloning bias due to transcription of toxic genes.
  • Easy-to-use: eliminates screening of recombinants due to its low background (4%).
  • Fast protocol: ligation time from 60 minutes to overnight.
  • Compatible: with direct cloning of PCR products.
  • Great versatility: compatible with any competent cell, primer design or particular proofreading DNA Polymerase.
  • Cost avoidance: avoids primer phosphorylation.
  • Risk-free: product covered by our Quality 100% Guarantee.

 

Download Documentation

 Data Sheet: https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/08/pSpark-TA3.pdf

 MSDS: https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/08/MSDS-pSpark-DNA-Cloning-Kits7.pdf

Application
  • Cloning of non-proofreading PCR fragments.
  • Production of ssDNA.
  • Blue/white screening for recombinants.
  • In vitro transcription from T7/SP6 dual-opposed promoters.
Quality Control
  • Functional test using a 600 pb PCR fragment.
Storage
  • Shipped in: Gel pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Advise
  • Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above    -20 °C inactivates the enzyme.

pSpark® TA Done DNA Cloning Kit

DISCLAIMER:
For research use only. We do not sell to patients and pharmacies
Catalog ID
Product
Cas #
Size
Price
Qty
20 rxn
$440.00
Catalog ID
Product
Cas #
Size
Price
Qty
20 rxn
$440.00