CVX5α™ Chemically Competent Cells

• Versatile: proven performance for high-efficiency transformation in a wide variety of applications.
• Convenient: ideal for routine.
• Compatible: with blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal.
• Cost avoidance: dry ice free of charge.

CVX5α™ Genotype:  F – , gyrA96, recA1, endA1, thi1, hsdR17 (rK – mK +), deoR, supE44, ∆ (lacZYA-argF) U169 φ80lacZ∆M15 (similar to DH5™ ).

 Manual: https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/08/COMPETENT-CELLS-CANVAX-REV-WEB.pdf

 Brochure: https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/08/pSpark%C2%AE-Universal-DNA-Cloning-kit-CVX5%CE%B1%E2%84%A2-Chemically-Competent-Cells1.pdf  

• Routine cloning and subcloning of genes into plasmid vectors.

• Each lot of competent cells is tested to verify transformation efficiencies using 100 pg pUC18 supercoiled DNA and the recommended protocol.
• Under these conditions, transformation efficiency will be ≥ 1 x 10⁷ cfu/μg pUC18.
• Transformation efficiency is defined as the number of colony forming units (cfu) produced by transforming 1 μg of plasmid (3 kb) into a given volume of competent cells.

• Shipped in: Dry ice.
• Storage: Upon receipt, store cells immediately at -80 °C.
• Recommendations: Storage at -20 °C will result in a significant decrease in transformation efficiency. Please note that competent cells are very sensitive to cycles of freezing and thawing and should not be exposed to temperature variations
• Guaranteed: 12 months from the date of purchase, if it is properly stored.

• Note: Optimal competence for cloning but it is not enough for the generation of cDNA libraries.