• Proteinase k is useful in the isolation of the DNA and RNA.

• Useful in Isolation of high molecular weight nucleic acid.

• Improve cloning efficiency of PCR products.

• Suitable for isolating PCR and RT-PCR templates.

• Proteinase k is useful for general protein digestion, used to digest endogenous DNases and

  RNases.

•One unit is defined as the amount of enzyme that will liberate 1.0 μmol

tyrosine (Folin-positive amino acid) from casein per minute at 37°C, pH 7.5

 •Active site: Active-site catalytic triad (Asp39-His69-Ser224)

 •Cleavage site: Peptide bonds adjacent to the carboxy group of

aliphatic aromatic amino acids with blocked alpha amino group.

• Specific Activity: ≥30 u/mg

•DNase activity: None

•RNase activity: None