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| Description |
pMBL™ T-Vector DNA Cloning Kit
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| Catalog No. |
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| Advantages & Features |
- Highly efficient: greater than 90% white colonies in a transformation with supplied insert control.
- Proven performance: more than 1,000 recombinant colonies expected in optimal conditions.
- Fast and easy protocol: results from 15 min protocol.
- Optimized: improve efficiency of ligation of a PCR product into the plasmid.
- Compatible: overhang for ligation of PCR products preventing recircularization of the vector.
- Designed by cutting the vector with EcoRV and adding a 3´ terminal thymidine to both ends.
- Risk-free: product covered by our Quality 100% Guarantee.
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| Download Documentation |
Manual: https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/08/pMBL-T-vector.pdf
MSDS: https://lifescience.canvaxbiotech.com/wp-content/uploads/sites/2/2015/08/pMBL%E2%84%A2-T-Vector-DNA-Cloning-Kit-Data-Sheet.pdf
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Application
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- Cloning of PCR fragments into DNA Cloning vector.
- Blue/white screening for recombinants.
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Quality Control
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- Functional test using a 600 pb PCR fragment.
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Storage
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- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
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Safety Statements
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This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website. |
Advise
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- Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above -20 °C inactivates the enzyme.
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